Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation

We investigated the in vitro GTPase activity of the S.cerevisae GTPase Cdc42, alone and in combination with its GDP/GTP exchange factor (GEF) Cdc24. We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data (amount of remaining GTP for various time points and protein concentrations) for variously tagged Cdc42 constructs and purification batches.