Supplementary data for the paper: Simultaneous orientation and 3D localization microscopy with a Vortex point spread function

This dataset contains data collected as part of the development of the Vortex PSF. It consists of raw data to calibrate the aberrations over the field-of-view, verify the vortex PSF model, visualise transitions in orientation and perform Binding-Activated localization microscopy and orientation estimation on lambda-DNA. A drift corrected and non drift corrected processed lambda-DNA dataset is also included to show the possible parameters that one can visualize over the whole field-of-view 

contributor: TU Delft, Faculty of Applied Sciences, Department of Imaging Physics