β-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana

Eva Liebminger, Christiane Veit, Martin Pabst, Martine Batoux, Cyril Zipfel, Friedrich Altmann, Lukas Mach, Richard Strasser

Research output: Contribution to journalArticleScientificpeer-review

51 Citations (Scopus)

Abstract

Most plant glycoproteins contain substantial amounts of paucimannosidic N-glycans instead of their direct biosynthetic precursors, complex N-glycans with terminal N-acetylglucosamine residues. We now demonstrate that two β-N-acetyl-hexosaminidases (HEXO1 and HEXO3) residing in different subcellular compartments jointly account for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. Total N-glycan analysis of hexo knock-out plants revealed that HEXO1 and HEXO3 contribute equally to the production of paucimannosidic N-glycans in roots, whereas N-glycan processing in leaves depends more heavily on HEXO3 than on HEXO1. Because hexo1 hexo3 double mutants do not display any obvious phenotype even upon exposure to different forms of abiotic or biotic stress, it should be feasible to improve the quality of glycoprotein therapeutics produced in plants by down-regulation of endogenous β-N-acetylhexosaminidase activities.

Original languageEnglish
Pages (from-to)10793-10802
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number12
DOIs
Publication statusPublished - 25 Mar 2011

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