3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells

Nastaran Barin, Hayri E. Balcioglu, Iris de Heer, Maurice de Wit, Martine L.M. Lamfers, Martin E. van Royen, Pim J. French*, Angelo Accardo*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)
37 Downloads (Pure)

Abstract

A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.

Original languageEnglish
Article number2204485
Number of pages14
JournalSmall
Volume18
Issue number49
DOIs
Publication statusPublished - 2022

Keywords

  • 2-photon polymerization
  • 3D cell culture
  • brain cancer
  • epidermal growth factor receptor
  • microtubes

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