3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

Jos de Wit; Sebastian Tonn; Mon-Ray Shao; Guido Van den Ackerveken; Jeroen Kalkman

doi:10.1364/3D.2024.JTh2A.16

3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

Jos de Wit, Sebastian Tonn, Mon-Ray Shao, Guido Van den Ackerveken, Jeroen Kalkman^*

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

Original language	English
Number of pages	2
DOIs	https://doi.org/10.1364/3D.2024.JTh2A.16
Publication status	Published - 2024
Event	Computational Optical Sensing and Imaging 2024 - Part of Optica Imaging Congress - Toulouse, France Duration: 15 Jul 2024 → 19 Jul 2024

Conference

Conference	Computational Optical Sensing and Imaging 2024 - Part of Optica Imaging Congress
Abbreviated title	COSI 2024
Country/Territory	France
City	Toulouse
Period	15/07/24 → 19/07/24

Bibliographical note

Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care
Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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10.1364/3D.2024.JTh2A.16

Cite this

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title = "3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography",

abstract = "Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.",

author = "{de Wit}, Jos and Sebastian Tonn and Mon-Ray Shao and {Van den Ackerveken}, Guido and Jeroen Kalkman",

note = "Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.; Computational Optical Sensing and Imaging 2024 - Part of Optica Imaging Congress, COSI 2024 ; Conference date: 15-07-2024 Through 19-07-2024",

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doi = "10.1364/3D.2024.JTh2A.16",

language = "English",

}

de Wit, J, Tonn, S, Shao, M-R, Van den Ackerveken, G & Kalkman, J 2024, '3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography', Paper presented at Computational Optical Sensing and Imaging 2024 - Part of Optica Imaging Congress, Toulouse, France, 15/07/24 - 19/07/24. https://doi.org/10.1364/3D.2024.JTh2A.16

TY - CONF

T1 - 3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

AU - de Wit, Jos

AU - Tonn, Sebastian

AU - Shao, Mon-Ray

AU - Van den Ackerveken, Guido

AU - Kalkman, Jeroen

N1 - Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

PY - 2024

Y1 - 2024

N2 - Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

AB - Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.

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Y2 - 15 July 2024 through 19 July 2024

ER -

3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography

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