Abstract
We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.
Original language | English |
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Title of host publication | Proceedings of the 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS) |
Place of Publication | Danvers |
Publisher | IEEE |
Pages | 102-105 |
Number of pages | 4 |
ISBN (Electronic) | 978-1-6654-0911-7 |
ISBN (Print) | 978-1-6654-0912-4 |
DOIs | |
Publication status | Published - 2022 |
Event | 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS) - Hybrid at Tokyo, Japan Duration: 9 Jan 2022 → 13 Jan 2022 Conference number: 35th |
Conference
Conference | 2022 IEEE 35th International Conference on Micro Electro Mechanical Systems Conference (MEMS) |
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Abbreviated title | MEMS 2022 |
Country/Territory | Japan |
City | Hybrid at Tokyo |
Period | 9/01/22 → 13/01/22 |
Bibliographical note
Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-careOtherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
Keywords
- brain-on-chip
- electrodes
- electrophysiology
- microelectrode array
- microfabrication
- neurons
- organ-on-chip