A compact microscope for voltage imaging

Xin Meng; Lex Huismans; Teun Huijben; Greta Szabo; Ruud Van Tol; Izak De Heer; Srividya Ganapathy; Daan Brinks

doi:10.1088/2040-8986/ac5dd5

A compact microscope for voltage imaging

Xin Meng, Lex Huismans, Teun Huijben, Greta Szabo, Ruud Van Tol, Izak De Heer, Srividya Ganapathy, Daan Brinks^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

2 Citations (Scopus)

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Abstract

Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

Original language	English
Article number	054004
Journal	Journal of Optics (United Kingdom)
Volume	24
Issue number	5
DOIs	https://doi.org/10.1088/2040-8986/ac5dd5
Publication status	Published - 2022

Keywords

electrophysiology
microscopy
neuroscience

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10.1088/2040-8986/ac5dd5

Meng_2022_J._Opt._24_054004Final published version, 2.32 MBLicence: CC BY

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AU - Meng, Xin

AU - Huismans, Lex

AU - Huijben, Teun

AU - Szabo, Greta

AU - Van Tol, Ruud

AU - De Heer, Izak

AU - Ganapathy, Srividya

AU - Brinks, Daan

PY - 2022

Y1 - 2022

N2 - Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

AB - Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

KW - electrophysiology

KW - microscopy

KW - neuroscience

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U2 - 10.1088/2040-8986/ac5dd5

DO - 10.1088/2040-8986/ac5dd5

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JO - Journal of Optics (United Kingdom)

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IS - 5

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ER -

A compact microscope for voltage imaging

Abstract

Keywords

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