A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Holly A. Reeve; Jake  Nicholson; Farieha  Altaf; Thomas H.  Lonsdale; Janina  Preissler; Lars Lauterbach; Oliver  Lenz; Silke  Leimkühler; F. Hollmann; C.E. Paul; Kylie A. Vincent

doi:10.1039/D2CC02411J

A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Holly A. Reeve^*, Jake Nicholson, Farieha Altaf, Thomas H. Lonsdale, Janina Preissler, Lars Lauterbach, Oliver Lenz, Silke Leimkühler, F. Hollmann, C.E. Paul^*, Kylie A. Vincent^*

^*Corresponding author for this work

BT/Biocatalysis

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

Original language	English
Pages (from-to)	10540-10543
Journal	Chemical Communications
Volume	58
Issue number	75
DOIs	https://doi.org/10.1039/D2CC02411J
Publication status	Published - 2022

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10.1039/D2CC02411J

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title = "A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H",

abstract = "We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.",

author = "Reeve, {Holly A.} and Jake Nicholson and Farieha Altaf and Lonsdale, {Thomas H.} and Janina Preissler and Lars Lauterbach and Oliver Lenz and Silke Leimk{\"u}hler and F. Hollmann and C.E. Paul and Vincent, {Kylie A.}",

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language = "English",

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T1 - A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

AU - Reeve, Holly A.

AU - Nicholson, Jake

AU - Altaf, Farieha

AU - Lonsdale, Thomas H.

AU - Preissler, Janina

AU - Lauterbach, Lars

AU - Lenz, Oliver

AU - Leimkühler, Silke

AU - Hollmann, F.

AU - Paul, C.E.

AU - Vincent, Kylie A.

PY - 2022

Y1 - 2022

N2 - We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

AB - We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

UR - http://www.scopus.com/inward/record.url?scp=85138457723&partnerID=8YFLogxK

U2 - 10.1039/D2CC02411J

DO - 10.1039/D2CC02411J

M3 - Article

SN - 1359-7345

VL - 58

SP - 10540

EP - 10543

JO - Chemical Communications

JF - Chemical Communications

IS - 75

ER -

A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Abstract

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