A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

Holly A. Reeve*, Jake Nicholson, Farieha Altaf, Thomas H. Lonsdale, Janina Preissler, Lars Lauterbach, Oliver Lenz, Silke Leimkühler, F. Hollmann, C.E. Paul*, Kylie A. Vincent*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.
Original languageEnglish
Pages (from-to)10540-10543
JournalChemical Communications
Volume58
Issue number75
DOIs
Publication statusPublished - 2022

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