A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring

N. Gaio*, A. Waafi, M.L.H. Vlaming, E. Boschman, P. Dijkstra, O. Nacken, S.R. Braam, C. Boucsein, P. M. Sarro, R. Dekker

*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

4 Citations (Scopus)
8 Downloads (Pure)

Abstract

This work presents the first multi-well plate that allows for simultaneous mechanical stimulation and electrical monitoring of multiple in-vitro cell cultures in parallel. Each well of the plate is equipped with an Organ-on-Chip (OOC) device consisting of a stretchable micro-electrode array (MEA). For the first time, a film assisted molding (FAM) process was employed to embed an OOC into a multi well plate format packaging. The functionality of the MEA in the device was assessed with electrochemical impedance spectroscopy. Moreover, the biocompatibility of the plate was demonstrated with cardiomyocytes derived from human induced pluripotent stem cells (iPSC) cultured in the wells.

Original languageEnglish
Title of host publication2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
Place of PublicationPiscataway, NJ
PublisherIEEE
Pages314-317
Number of pages4
Volume2018-January
ISBN (Electronic)978-1-5386-4782-0
DOIs
Publication statusPublished - 2018
EventMEMS 2018: 31st IEEE International Conference on Micro Electro Mechanical Systems - Belfast, United Kingdom
Duration: 21 Jan 201825 Jan 2018
https://www.mems2018.org/

Conference

ConferenceMEMS 2018
CountryUnited Kingdom
CityBelfast
Period21/01/1825/01/18
Internet address

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care
Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Keywords

  • Electrodes
  • Monitoring
  • Silicon
  • Packaging
  • Biomedical monitoring
  • Biomembranes
  • Impedance

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