TY - JOUR
T1 - A Novel Unspecific Peroxygenase from Galatian marginata for Biocatalytic Oxyfunctionalization Reactions
AU - Ma, Yunjian
AU - Liang, Hongjing
AU - Zhao, Zexin
AU - Wu, Bin
AU - Lan, Dongming
AU - Hollmann, Frank
AU - Wang, Yonghua
PY - 2022
Y1 - 2022
N2 - Unspecific peroxygenases (UPOs, EC 1.11.2.1) are promising oxyfunctionalization catalysts because of their unique stereoselectivity. However, so far only a few UPOs have been reported. In this study, gene mining was used to identify a gene from Galerina marginata that coded for a novel UPO (GmaUPO). GmaUPO was expressed in Pichia pastoris X-33 by scale-up fermentation (the UPO activity of the culture supernatant was 118 U/L). GmaUPO exhibited a molecular weight of 40 kDa and exhibited highest activity at 35°C and pH 9, respectively. Furthermore, GmaUPO was demonstrated to catalyze the epoxidation, sulfoxidation, and hydroxylation of common substrates, particularly fatty acids such as tridecanoic acid. The molecular basis for GmaUPO regioselectivity for fatty acid hydroxylation was explored by molecular modelling. The regioselectivity was mostly governed by the architecture of the enzyme's active site.
AB - Unspecific peroxygenases (UPOs, EC 1.11.2.1) are promising oxyfunctionalization catalysts because of their unique stereoselectivity. However, so far only a few UPOs have been reported. In this study, gene mining was used to identify a gene from Galerina marginata that coded for a novel UPO (GmaUPO). GmaUPO was expressed in Pichia pastoris X-33 by scale-up fermentation (the UPO activity of the culture supernatant was 118 U/L). GmaUPO exhibited a molecular weight of 40 kDa and exhibited highest activity at 35°C and pH 9, respectively. Furthermore, GmaUPO was demonstrated to catalyze the epoxidation, sulfoxidation, and hydroxylation of common substrates, particularly fatty acids such as tridecanoic acid. The molecular basis for GmaUPO regioselectivity for fatty acid hydroxylation was explored by molecular modelling. The regioselectivity was mostly governed by the architecture of the enzyme's active site.
KW - Bioinformatics analysis
KW - Galerina marginata
KW - Heterologous expression
KW - Oxyfunctionalization
KW - Unspecific peroxygenase
UR - http://www.scopus.com/inward/record.url?scp=85139082747&partnerID=8YFLogxK
U2 - 10.1016/j.mcat.2022.112707
DO - 10.1016/j.mcat.2022.112707
M3 - Article
AN - SCOPUS:85139082747
SN - 2468-8231
VL - 531
JO - Molecular Catalysis
JF - Molecular Catalysis
ER -