A Photoenzymatic NADH Regeneration System

Georg T. Höfler, Elena Fernández-Fueyo, Milja Pesic, Sabry H. Younes, Eun Gyu Choi, Yong H. Kim, Vlada B. Urlacher, Isabel W.C.E. Arends, Frank Hollmann*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)
47 Downloads (Pure)

Abstract

A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.

Original languageEnglish
Number of pages5
JournalChemBioChem
Volume19
DOIs
Publication statusPublished - 2018

Keywords

  • biocatalysis
  • enantioselectivity
  • ketones
  • photochemistry
  • reduction

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