TY - JOUR
T1 - Acid phosphatase behaviour at an electrified soft junction and its interfacial co-deposition with silica
AU - Poltorak, Lukasz
AU - van der Meijden, Nienke
AU - Oonk, Stijn
AU - Sudhölter, Ernst J.R.
AU - de Puit, Marcel
PY - 2018/9/1
Y1 - 2018/9/1
N2 - The behaviour of acid phosphatase at an electrified liquid–liquid interface was studied in this work. It was found that only the protonated form of the protein can undergo interfacial adsorption which is affected by the pH of the aqueous phase. With ion transfer voltammetry we could detect acid phosphatase in concentrations as low as 0.1 μM. We were able to co-deposit the protein and silica at the electrified liquid–liquid interface via controlled proton transfer to the organic phase where it catalyzed tetraethoxysilane hydrolysis, followed by polycondensation to silica.
AB - The behaviour of acid phosphatase at an electrified liquid–liquid interface was studied in this work. It was found that only the protonated form of the protein can undergo interfacial adsorption which is affected by the pH of the aqueous phase. With ion transfer voltammetry we could detect acid phosphatase in concentrations as low as 0.1 μM. We were able to co-deposit the protein and silica at the electrified liquid–liquid interface via controlled proton transfer to the organic phase where it catalyzed tetraethoxysilane hydrolysis, followed by polycondensation to silica.
KW - Acid phosphatase
KW - Interfacial deposition
KW - ITIES
KW - Proteins
KW - Sol-gel process of silica
UR - http://resolver.tudelft.nl/uuid:928b9f13-50d5-4274-a4d7-575604594cad
UR - http://www.scopus.com/inward/record.url?scp=85050726974&partnerID=8YFLogxK
U2 - 10.1016/j.elecom.2018.07.022
DO - 10.1016/j.elecom.2018.07.022
M3 - Article
AN - SCOPUS:85050726974
SN - 1388-2481
VL - 94
SP - 27
EP - 30
JO - Electrochemistry Communications
JF - Electrochemistry Communications
ER -