Acid phosphatase behaviour at an electrified soft junction and its interfacial co-deposition with silica

Lukasz Poltorak, Nienke van der Meijden, Stijn Oonk, Ernst J.R. Sudhölter, Marcel de Puit

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)
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Abstract

The behaviour of acid phosphatase at an electrified liquid–liquid interface was studied in this work. It was found that only the protonated form of the protein can undergo interfacial adsorption which is affected by the pH of the aqueous phase. With ion transfer voltammetry we could detect acid phosphatase in concentrations as low as 0.1 μM. We were able to co-deposit the protein and silica at the electrified liquid–liquid interface via controlled proton transfer to the organic phase where it catalyzed tetraethoxysilane hydrolysis, followed by polycondensation to silica.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalElectrochemistry Communications
Volume94
DOIs
Publication statusPublished - 1 Sep 2018

Keywords

  • Acid phosphatase
  • Interfacial deposition
  • ITIES
  • Proteins
  • Sol-gel process of silica

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