Actin networks regulate the cell membrane permeability during electroporation

Research output: Contribution to journalArticleScientificpeer-review

3 Downloads (Pure)

Abstract

Transient physical disruption of cell membranes by electric pulses (or electroporation) has significance in biomedical and biological applications requiring the delivery of exogenous (bio)molecules to living cells. We demonstrate that actin networks regulate the cell membrane permeability during electroporation. Disruption of actin networks increases the uptake of membrane-impermeable molecules such as propidium iodide during electroporation. Our experiments at different temperatures ranging from 11 °C to 37 °C show that molecular uptake during electroporation increases with temperature. Furthermore, by examining the temperature-dependent kinetics of propidium iodide uptake, we infer that the activation energy barrier of electroporation is lowered when the actin networks are disrupted. Our numerical calculations of transmembrane voltage show that the reduced activation energy barrier for the cells with disrupted actin is not a consequence of the changes in transmembrane voltage associated with changes in the cell shape due to the disruption of actin, indicating that this could be due to changes in membrane mechanical properties. Our results suggest that the current theoretical models of electroporation should be advanced further by including the contributions of the cytoskeletal networks on the cell membrane permeability during the delivery of exogenous materials.

Original languageEnglish
Article number183468
Pages (from-to)183468
Number of pages1
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1863
Issue number1
DOIs
Publication statusPublished - 2021

Keywords

  • Electroporation
  • Electropermeabilization
  • Actin networks
  • Energy barrier
  • Temperature dependent kinetics of electroporation

Fingerprint Dive into the research topics of 'Actin networks regulate the cell membrane permeability during electroporation'. Together they form a unique fingerprint.

  • Cite this