Adaptive optics for confocal laser scanning microscopy with adjustable pinhole

H.W. Yoo, M.E. van Royen, WA van Cappellen, AB Houtsmuller, Michel Verhaegen, G Schitter

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

3 Citations (Scopus)
252 Downloads (Pure)

Abstract

The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with such a trade-off. The proposed adjustable pinhole enables to calibrate the AO system and to evaluate the imaging performance. Experimental results with fluorescence beads on the coverslip and at a depth of 40 μm in the human hepatocellular carcinoma cell spheroid demonstrate that the proposed AO system can improve the image quality by the proposed calibration method. The proposed pinhole intensity ratio also indicates the image improvement by the AO correction in intensity as well as resolution.
Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationBiophotonics: Photonic Solutions for Better Health Care V
EditorsJürgen Popp, Valery V. Tuchin, Dennis L. Matthews, Francesco S. Pavone
Place of PublicationBellingham, WA, USA
PublisherSPIE
Number of pages11
Volume9887
ISBN (Print)978-1-510601321
DOIs
Publication statusPublished - 2016
EventBiophotonics: Photonic Solutions for Better Health Care V - Brussels, Belgium
Duration: 4 Apr 20167 Apr 2016

Publication series

NameProceedings of SPIE
Volume9887
ISSN (Electronic)1605-7422

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care V
CountryBelgium
CityBrussels
Period4/04/167/04/16

Keywords

  • Adaptive Optics
  • Square Adjustable Pinhole
  • Confocal Laser Scanning Microscopy
  • Pinhole Intensity Ratio

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