Adaptive optics in digital micromirror based confocal microscopy

Paolo Pozzi, Dean Wilding, Oleg Soloviev, Gleb Vdovine, Michel Verhaegen

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Abstract

This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in astronomical imaging, adaptive optics is achieved through a closed loop in which the actuators of a deformable mirror are driven by a wavefront sensor. This approach is severely limited in fluorescence microscopy, as the use of a wavefront sensor requires the presence of a bright, point like source in the field of view, a condition rarely satisfied in microscopy samples. Previously reported approaches to adaptive optics in fluorescence microscopy are therefore limited to the inclusion of fluorescent microspheres in the sample, to use as bright stars for wavefront sensors, or time consuming sensorless optimization procedures, requiring several seconds of optimization before the acquisition of a single image.

We propose an alternative approach to the problem, implementing sensorless adaptive optics in a Programmable array microscope. A programmable array microscope is a microscope based on a digital micromirror device, in which the single elements of the micromirror act both as point sources and pinholes.
Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationAdaptive Optics and Wavefront Control for Biological Systems II
EditorsThomas G. Bifano, Joel Kubby, Sylvain Gigan
Place of PublicationBellingham, WA, USA
PublisherSPIE
Number of pages7
Volume9717
ISBN (Print)978-1-628419511
DOIs
Publication statusPublished - 2016
EventAdaptive Optics and Wavefront Control for Biological Systems II - San Francisco, United States
Duration: 13 Feb 201615 Feb 2016

Publication series

NameProceedings of SPIE
Volume9717
ISSN (Electronic)1605-7422

Conference

ConferenceAdaptive Optics and Wavefront Control for Biological Systems II
CountryUnited States
CitySan Francisco
Period13/02/1615/02/16

Keywords

  • Confocal Microscopy
  • Digital Micromirror Device
  • Wavefront Optimization
  • Fluorescence

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    Pozzi, P., Wilding, D., Soloviev, O., Vdovine, G., & Verhaegen, M. (2016). Adaptive optics in digital micromirror based confocal microscopy. In T. G. Bifano, J. Kubby, & S. Gigan (Eds.), Proceedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems II (Vol. 9717). [971706] (Proceedings of SPIE; Vol. 9717). SPIE. https://doi.org/10.1117/12.2208331