Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

S. Hung; Arnau  Llobet Rosell; Daphne Jurriens; O.A. Soloviev; Lukas C. Kapitein; K.S. Grußmayer; Lukas J.  Neukomm; M.H.G. Verhaegen; C.S. Smith

doi:10.3389/fnins.2022.954949

Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

S. Hung, Arnau Llobet Rosell, Daphne Jurriens, O.A. Soloviev, Lukas C. Kapitein, K.S. Grußmayer, Lukas J. Neukomm, M.H.G. Verhaegen, C.S. Smith^*

^*Corresponding author for this work

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Abstract

Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

Original language	English
Article number	954949
Number of pages	21
Journal	Frontiers in Neuroscience
Volume	16
DOIs	https://doi.org/10.3389/fnins.2022.954949
Publication status	Published - 2022

Keywords

Super-resolution Microscopy
localization microscopy
adaptive optics
Drosophila
brain

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Hung, S., Llobet Rosell, A., Jurriens, D., Soloviev, O. A., Kapitein, L. C., Grußmayer, K. S., Neukomm, L. J., Verhaegen, M. H. G., & Smith, C. S. (2022). Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains. Frontiers in Neuroscience, 16, Article 954949. https://doi.org/10.3389/fnins.2022.954949

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title = "Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains",

abstract = "Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.",

keywords = "Super-resolution Microscopy, localization microscopy, adaptive optics, Drosophila, brain",

author = "S. Hung and {Llobet Rosell}, Arnau and Daphne Jurriens and O.A. Soloviev and Kapitein, {Lukas C.} and K.S. Gru{\ss}mayer and Neukomm, {Lukas J.} and M.H.G. Verhaegen and C.S. Smith",

year = "2022",

doi = "10.3389/fnins.2022.954949",

language = "English",

volume = "16",

journal = "Frontiers in Neuroscience",

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publisher = "Frontiers Media",

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TY - JOUR

T1 - Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

AU - Hung, S.

AU - Llobet Rosell, Arnau

AU - Jurriens, Daphne

AU - Soloviev, O.A.

AU - Kapitein, Lukas C.

AU - Grußmayer, K.S.

AU - Neukomm, Lukas J.

AU - Verhaegen, M.H.G.

AU - Smith, C.S.

PY - 2022

Y1 - 2022

N2 - Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

AB - Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.

KW - Super-resolution Microscopy

KW - localization microscopy

KW - adaptive optics

KW - Drosophila

KW - brain

U2 - 10.3389/fnins.2022.954949

DO - 10.3389/fnins.2022.954949

M3 - Article

SN - 1662-4548

VL - 16

JO - Frontiers in Neuroscience

JF - Frontiers in Neuroscience

M1 - 954949

ER -

Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

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Keywords

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