TY - JOUR
T1 - Adsorption Sequencing as a Rapid Method to Link Environmental Bacteriophages to Hosts
AU - de Jonge, Patrick A.
AU - von Meijenfeldt, F. A.Bastiaan
AU - Costa, Ana Rita
AU - Nobrega, Franklin L.
AU - Brouns, Stan J.J.
AU - Dutilh, Bas E.
PY - 2020
Y1 - 2020
N2 - An important viromics challenge is associating bacteriophages to hosts. To address this, we developed adsorption sequencing (AdsorpSeq), a readily implementable method to measure phages that are preferentially adsorbed to specific host cell envelopes. AdsorpSeq thus captures the key initial infection cycle step. Phages are added to cell envelopes, adsorbed phages are isolated through gel electrophoresis, after which adsorbed phage DNA is sequenced and compared with the full virome. Here, we show that AdsorpSeq allows for separation of phages based on receptor-adsorbing capabilities. Next, we applied AdsorpSeq to identify phages in a wastewater virome that adsorb to cell envelopes of nine bacteria, including important pathogens. We detected 26 adsorbed phages including common and rare members of the virome, a minority being related to previously characterized phages. We conclude that AdsorpSeq is an effective new tool for rapid characterization of environmental phage adsorption, with a proof-of-principle application to Gram-negative host cell envelopes.
AB - An important viromics challenge is associating bacteriophages to hosts. To address this, we developed adsorption sequencing (AdsorpSeq), a readily implementable method to measure phages that are preferentially adsorbed to specific host cell envelopes. AdsorpSeq thus captures the key initial infection cycle step. Phages are added to cell envelopes, adsorbed phages are isolated through gel electrophoresis, after which adsorbed phage DNA is sequenced and compared with the full virome. Here, we show that AdsorpSeq allows for separation of phages based on receptor-adsorbing capabilities. Next, we applied AdsorpSeq to identify phages in a wastewater virome that adsorb to cell envelopes of nine bacteria, including important pathogens. We detected 26 adsorbed phages including common and rare members of the virome, a minority being related to previously characterized phages. We conclude that AdsorpSeq is an effective new tool for rapid characterization of environmental phage adsorption, with a proof-of-principle application to Gram-negative host cell envelopes.
KW - Ecology
KW - Environmental Science
KW - Microbiology
KW - Techniques in Genetics
UR - http://www.scopus.com/inward/record.url?scp=85089502743&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2020.101439
DO - 10.1016/j.isci.2020.101439
M3 - Article
AN - SCOPUS:85089502743
SN - 2589-0042
VL - 23
JO - iScience
JF - iScience
IS - 9
M1 - 101439
ER -