TY - JOUR
T1 - An Efficient Strategy for the Production of Epoxidized Oils
T2 - Natural Deep Eutectic Solvent-Based Enzymatic Epoxidation
AU - Zhang, Tianyu
AU - Ma, Yunjian
AU - Tan, Chin Ping
AU - Hollmann, Frank
AU - Wang, Jianrong
AU - Yang, Bo
AU - Wang, Yonghua
N1 - Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
PY - 2019
Y1 - 2019
N2 -
Poor H
2
O
2
-resistance by enzymes is a key bottleneck in the epoxidation process of oil by enzymatic methods. In this study, the stability of three lipases, from Aspergillus oryzae lipase (AOL), Aspergillus fumigatus lipase B (AflB), and marine Janibacter (MAJ1), in the presence of H
2
O
2
was evaluated in different types of natural deep eutectic solvents (NADES). This stability was strengthened significantly in the NADES compared to the buffer. Specifically, AOL retained 84.7% of its initial activity in the presence of choline chloride/sorbitol (1:1 M ratio) and 3 mol L
−1
H
2
O
2
after 24 h incubation at 40°C. In addition, the two-phase epoxidation process was optimized with AOL in ChCl/sorbitol to reach up to 96.8% conversion under the optimized conditions (molar ratio of octanoic acid/H
2
O
2
/C=C-bonds = 0.3:1.5:1, enzyme loading of 15 U g
−1
of soybean oil, ChCl/sorbitol content of 70.0% of the weight of hydrophilic phase, and reaction temperature of 50°C). Moreover, the lipase dispersed in NADES retained approximately 66% of its initial activity after being used for seven batch cycles. Overall, NADES-based enzymatic epoxidation is a feasible and promising strategy for the synthesis of epoxidized oils.
AB -
Poor H
2
O
2
-resistance by enzymes is a key bottleneck in the epoxidation process of oil by enzymatic methods. In this study, the stability of three lipases, from Aspergillus oryzae lipase (AOL), Aspergillus fumigatus lipase B (AflB), and marine Janibacter (MAJ1), in the presence of H
2
O
2
was evaluated in different types of natural deep eutectic solvents (NADES). This stability was strengthened significantly in the NADES compared to the buffer. Specifically, AOL retained 84.7% of its initial activity in the presence of choline chloride/sorbitol (1:1 M ratio) and 3 mol L
−1
H
2
O
2
after 24 h incubation at 40°C. In addition, the two-phase epoxidation process was optimized with AOL in ChCl/sorbitol to reach up to 96.8% conversion under the optimized conditions (molar ratio of octanoic acid/H
2
O
2
/C=C-bonds = 0.3:1.5:1, enzyme loading of 15 U g
−1
of soybean oil, ChCl/sorbitol content of 70.0% of the weight of hydrophilic phase, and reaction temperature of 50°C). Moreover, the lipase dispersed in NADES retained approximately 66% of its initial activity after being used for seven batch cycles. Overall, NADES-based enzymatic epoxidation is a feasible and promising strategy for the synthesis of epoxidized oils.
KW - Enzyme catalysis
KW - Epoxidation
KW - Lipase
KW - Natural deep eutectic solvent
KW - Soybean oil
UR - http://www.scopus.com/inward/record.url?scp=85065156357&partnerID=8YFLogxK
U2 - 10.1002/aocs.12220
DO - 10.1002/aocs.12220
M3 - Article
AN - SCOPUS:85065156357
VL - 96
SP - 671
EP - 679
JO - JAOCS: Journal of the American Oil Chemists' Society
JF - JAOCS: Journal of the American Oil Chemists' Society
SN - 0003-021X
IS - 6
ER -