TY - JOUR
T1 - Application of ultraviolet, visible, and infrared light imaging in protein-based biopharmaceutical formulation characterization and development studies
AU - Klijn, M.E.
AU - Hubbuch, Juergen
PY - 2021
Y1 - 2021
N2 - Imaging is increasingly more utilized as analytical technology in biopharmaceutical formulation research, with applications ranging from subvisible particle characterization to thermal stability screening and residual moisture analysis. This review offers a comprehensive overview of analytical imaging for scientists active in biopharmaceutical formulation research and development, where it presents the unique information provided by the ultraviolet (UV), visible (Vis), and infrared (IR) sections in the electromagnetic spectrum. The main body of this review consists of an outline of UV, Vis, and IR imaging techniques for several (bio)physical properties that are commonly determined during protein-based biopharmaceutical formulation characterization and development studies. The review concludes with a future perspective of applied imaging within the field of biopharmaceutical formulation research.
AB - Imaging is increasingly more utilized as analytical technology in biopharmaceutical formulation research, with applications ranging from subvisible particle characterization to thermal stability screening and residual moisture analysis. This review offers a comprehensive overview of analytical imaging for scientists active in biopharmaceutical formulation research and development, where it presents the unique information provided by the ultraviolet (UV), visible (Vis), and infrared (IR) sections in the electromagnetic spectrum. The main body of this review consists of an outline of UV, Vis, and IR imaging techniques for several (bio)physical properties that are commonly determined during protein-based biopharmaceutical formulation characterization and development studies. The review concludes with a future perspective of applied imaging within the field of biopharmaceutical formulation research.
KW - Computer vision
KW - Formulation development
KW - High-throughput screening
KW - Image processing
KW - Machine learning
KW - Protein analytics
UR - http://www.scopus.com/inward/record.url?scp=85107660254&partnerID=8YFLogxK
U2 - 10.1016/j.ejpb.2021.05.013
DO - 10.1016/j.ejpb.2021.05.013
M3 - Article
SN - 0939-6411
VL - 165
SP - 319
EP - 336
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
ER -