TY - JOUR
T1 - Approaches for bacteriophage genome engineering
AU - Mahler, Marina
AU - Costa, Ana Rita
AU - van Beljouw, Sam P.B.
AU - Fineran, Peter C.
AU - Brouns, Stan J.J.
PY - 2023
Y1 - 2023
N2 - In recent years, bacteriophage research has been boosted by a rising interest in using phage therapy to treat antibiotic-resistant bacterial infections. In addition, there is a desire to use phages and their unique proteins for specific biocontrol applications and diagnostics. However, the ability to manipulate phage genomes to understand and control gene functions, or alter phage properties such as host range, has remained challenging due to a lack of universal selectable markers. Here, we discuss the state-of-the-art techniques to engineer and select desired phage genomes using advances in cell-free methodologies and clustered regularly interspaced short palindromic repeats-CRISPR associated protein (CRISPR-Cas) counter-selection approaches.
AB - In recent years, bacteriophage research has been boosted by a rising interest in using phage therapy to treat antibiotic-resistant bacterial infections. In addition, there is a desire to use phages and their unique proteins for specific biocontrol applications and diagnostics. However, the ability to manipulate phage genomes to understand and control gene functions, or alter phage properties such as host range, has remained challenging due to a lack of universal selectable markers. Here, we discuss the state-of-the-art techniques to engineer and select desired phage genomes using advances in cell-free methodologies and clustered regularly interspaced short palindromic repeats-CRISPR associated protein (CRISPR-Cas) counter-selection approaches.
KW - CRISPR-Cas
KW - homologous recombination
KW - phage engineering
KW - rebooting
KW - recombineering
UR - http://www.scopus.com/inward/record.url?scp=85138583075&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2022.08.008
DO - 10.1016/j.tibtech.2022.08.008
M3 - Review article
C2 - 36117025
AN - SCOPUS:85138583075
SN - 0167-7799
VL - 41
SP - 669
EP - 685
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 5
ER -