TY - JOUR
T1 - Assessment of bacterial and structural dynamics in aerobic granular biofilms
AU - Weissbrodt, David G.
AU - Neu, Thomas R.
AU - Kuhlicke, Ute
AU - Rappaz, Yoan
AU - Holliger, Christof
PY - 2013
Y1 - 2013
N2 - Aerobic granular sludge (AGS) is based on self-granulated flocs forming mobile biofilms with a gel-like consistence. Bacterial and structural dynamics from flocs to granules were followed in anaerobic-aerobic sequencing batch reactors (SBR) fed with synthetic wastewater, namely a bubble column (BC-SBR) operated under wash-out conditions for fast granulation, and two stirred-tank enrichments of Accumulibacter (PAO-SBR) and Competibacter (GAO-SBR) operated at steady-state. In the BC-SBR, granules formed within 2 weeks by swelling of Zoogloea colonies around flocs, developing subsequently smooth zoogloeal biofilms. However, Zoogloea predominance (37-79%) led to deteriorated nutrient removal during the first months of reactor operation. Upon maturation, improved nitrification (80-100%), nitrogen removal (43-83%), and high but unstable dephosphatation (75-100%) were obtained. Proliferation of dense clusters of nitrifiers, Accumulibacter, and Competibacter from granule cores outwards resulted in heterogeneous bioaggregates, inside which only low abundance Zoogloea (
AB - Aerobic granular sludge (AGS) is based on self-granulated flocs forming mobile biofilms with a gel-like consistence. Bacterial and structural dynamics from flocs to granules were followed in anaerobic-aerobic sequencing batch reactors (SBR) fed with synthetic wastewater, namely a bubble column (BC-SBR) operated under wash-out conditions for fast granulation, and two stirred-tank enrichments of Accumulibacter (PAO-SBR) and Competibacter (GAO-SBR) operated at steady-state. In the BC-SBR, granules formed within 2 weeks by swelling of Zoogloea colonies around flocs, developing subsequently smooth zoogloeal biofilms. However, Zoogloea predominance (37-79%) led to deteriorated nutrient removal during the first months of reactor operation. Upon maturation, improved nitrification (80-100%), nitrogen removal (43-83%), and high but unstable dephosphatation (75-100%) were obtained. Proliferation of dense clusters of nitrifiers, Accumulibacter, and Competibacter from granule cores outwards resulted in heterogeneous bioaggregates, inside which only low abundance Zoogloea (
KW - Aerobic granular sludge
KW - Biological wastewater treatment
KW - CLSM
KW - FISH
KW - FLBA
KW - Granular biofilm formation and structure
KW - Pyrosequencing
KW - T-RFLP
UR - http://www.scopus.com/inward/record.url?scp=84885368203&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2013.00175
DO - 10.3389/fmicb.2013.00175
M3 - Article
AN - SCOPUS:84885368203
SN - 1664-302X
VL - 4
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - JUL
ER -