Asymmetric Reduction of (R)-Carvone through a Thermostable and Organic-Solvent-Tolerant Ene-Reductase

Dirk Tischler, Eric Gädke, Daniel Eggerichs, Alvaro Gomez Baraibar, Carolin Mügge, Anika Scholtissek, Caroline E. Paul

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Abstract

Ene-reductases allow regio- and stereoselective reduction of activated C=C double bonds at the expense of nicotinamide adenine dinucleotide cofactors [NAD(P)H]. Biological NAD(P)H can be replaced by synthetic mimics to facilitate enzyme screening and process optimization. The ene-reductase FOYE-1, originating from an acidophilic iron oxidizer, has been described as a promising candidate and is now being explored for applied biocatalysis. Biological and synthetic nicotinamide cofactors were evaluated to fuel FOYE-1 to produce valuable compounds. A maximum activity of (319.7±3.2) U mg−1 with NADPH or of (206.7±3.4) U mg−1 with 1-benzyl-1,4-dihydronicotinamide (BNAH) for the reduction of N-methylmaleimide was observed at 30 °C. Notably, BNAH was found to be a promising reductant but exhibits poor solubility in water. Different organic solvents were therefore assayed: FOYE-1 showed excellent performance in most systems with up to 20 vol% solvent and at temperatures up to 40 °C. Purification and application strategies were evaluated on a small scale to optimize the process. Finally, a 200 mL biotransformation of 750 mg (R)-carvone afforded 495 mg of (2R,5R)-dihydrocarvone (>95 % ee), demonstrating the simplicity of handling and application of FOYE-1.

Original languageEnglish
Pages (from-to)1217-1225
Number of pages9
JournalChemBioChem
Volume21
Issue number8
DOIs
Publication statusPublished - 2020

Keywords

  • biocatalysis
  • biotransformations
  • cofactor mimics
  • Old Yellow Enzymes
  • oxidoreductases
  • solvent stability

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    Tischler, D., Gädke, E., Eggerichs, D., Gomez Baraibar, A., Mügge, C., Scholtissek, A., & Paul, C. E. (2020). Asymmetric Reduction of (R)-Carvone through a Thermostable and Organic-Solvent-Tolerant Ene-Reductase. ChemBioChem, 21(8), 1217-1225. https://doi.org/10.1002/cbic.201900599