Abstract
Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.
Original language | English |
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Pages (from-to) | 4080-4086 |
Number of pages | 7 |
Journal | ChemCatChem |
Volume | 13 |
Issue number | 19 |
DOIs | |
Publication status | Published - 2021 |
Keywords
- biocatalysis
- decarboxylase
- hydratase
- nano-scale bioreactor
- outer membrane vesicles