Bacterial Outer Membrane Vesicles as Nano-Scale Bioreactors: A Fatty Acid Conversion Case Study

Ji Won Song, Yoonjin Baeg, Ha Yeon Jeong, Jinwon Lee, Deok Kun Oh, Frank Hollmann, Jin Byung Park*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the biotransformation of fatty acids by targeting a fatty acid double bond hydratase of Stentrophomonas maltophilia (SmOhyA) and/or a photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered OMVs containing both SmOhyA and CvFAP were able to catalyse the transformation of oleic acid ((Z)-octadec-9-enoic acid) into 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific biotransformation rates of oleic acid reached 8.0×10−12 μmol/min per OMV.

Original languageEnglish
Pages (from-to)4080-4086
Number of pages7
JournalChemCatChem
Volume13
Issue number19
DOIs
Publication statusPublished - 2021

Keywords

  • biocatalysis
  • decarboxylase
  • hydratase
  • nano-scale bioreactor
  • outer membrane vesicles

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