Benchmarking and developing mass-spectrometry based omics approaches for complex microbiomes

H.B.C. Kleikamp

Research output: ThesisDissertation (TU Delft)

75 Downloads (Pure)

Abstract

The large unexplored space of microbial biodiversity forms a well of untapped potential of enzymes and bioactive compounds. Genomic methods have made significant improvements over the past decades in their analysis of microbial communities. However, several aspects of nature can’t be described by a genome alone, such as expressed functions in the proteome and complex products such as bacterial glycosylation. Mass spectrometry can provide both general and targeted methods for the analysis of phenotypes, but is facing challenges in its limited throughput and the demanding data analysis of complex samples, which necessitates new methodologies. This thesis therefore aims to compare, improve and develop now methods for the analysis of microbial communities using mass spectrometry...
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Delft University of Technology
Supervisors/Advisors
  • van Loosdrecht, Mark C.M., Supervisor
  • Pabst, Martin, Advisor
  • Lin, Y., Advisor
Award date16 May 2022
DOIs
Publication statusPublished - 2022

Funding

Funded by Soenghen Institute of Anaerobic Microbiology (SIAM)

Keywords

  • mass spectrometry
  • proteomics
  • glycomics
  • metagenomics
  • sialic acids
  • Aerobic granular sludge
  • de novo peptide sequencing

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