Biliverdin-copper complex at physiological pH

Milena S. Dimitrijević, Jelena Bogdanović Pristov, Milan Žižić, Dalibor M. Stanković, Danica Bajuk-Bogdanović, Marina Stanić, Snežana Spasić, Wilfred Hagen, Ivan Spasojević

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Abstract

Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu 2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, 1 H NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1:1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e - . The delocalized electron may come from BV or Cu 2+ , so the complex is formally composed either of BV radical cation and Cu 1+ or of BV radical anion and Cu 3+ . The complex underwent oxidation only in the presence of both O 2 and an excess of Cu 2+ , or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.

Original languageEnglish
Pages (from-to)6061-6070
JournalDalton Transactions
Volume48
Issue number18
DOIs
Publication statusPublished - 2019

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    Dimitrijević, M. S., Bogdanović Pristov, J., Žižić, M., Stanković, D. M., Bajuk-Bogdanović, D., Stanić, M., Spasić, S., Hagen, W., & Spasojević, I. (2019). Biliverdin-copper complex at physiological pH. Dalton Transactions, 48(18), 6061-6070. https://doi.org/10.1039/c8dt04724c