In this chapter an overview will be given of the biotechnological possibilities for repair of concrete with focus on application of limestone-producing bacteria and the different metabolic pathways involved, e.g., via hydrolysis of urea and heterotrophic CO2 production under alkaline conditions. The first paragraph comprises an overview of previously published reports on this subject. In the two succeeding paragraphs, two specific systems for biotechnological repair of concrete structures will be discussed. The first one covers liquid biobased repair systems for durable repair of cracked and porous concrete surfaces, and the second one addresses biobased mortar systems for repair of larger defects of concrete structures. The cases discussed here indicate that concrete repair applying biotech solutions results in improved material durability that can save money and at the same time lower the environmental impact of civil engineering activities.