TY - JOUR
T1 - Co-regulation of substrate flux and biomass density for enhanced ammonia assimilation under saline conditions
AU - Zhao, Chuanfu
AU - Zhang, Wenhao
AU - Zhang, Shuhui
AU - Zhang, Lei
AU - Han, Fei
AU - Lei, Jianhua
AU - Li, Yuke
AU - Zhou, Weizhi
PY - 2026
Y1 - 2026
N2 - Drawing inspiration from the self-regulating carbon–nitrogen cycling of saline ecosystems, this study investigates how substrate flux and biomass density co-regulate the structure and function of marine sediment-derived halophilic heterotrophic ammonia assimilation (HAA) microbiome cultivated in saline ammonia-containing wastewater with a COD/N radio of 20 under volumetric exchange ratios (VER) of 75 %, 50 %, and 25 % and mixed liquor suspended solids (MLSS) increasing from 5 to 15 g/L. The combined variation in VER and MLSS generated a gradient in food-to-microorganism radio (F/M). With increasing in biomass, COD removal efficiencies peaked at 94.4–99.3 % at 15 g MLSS/L, whereas ammonia removal efficiencies reached at 90.3–96.8 % at 12.5 g MLSS/L before declining. A VER of 25 % reduced sludge activity, while a VER of 75 % impaired floc settleability. The directed HAA community shifted in substrate flux and biomass density, centering on dominant genera such as Halomonas and Marinobacter, ultimately forming a stable microbiome.
AB - Drawing inspiration from the self-regulating carbon–nitrogen cycling of saline ecosystems, this study investigates how substrate flux and biomass density co-regulate the structure and function of marine sediment-derived halophilic heterotrophic ammonia assimilation (HAA) microbiome cultivated in saline ammonia-containing wastewater with a COD/N radio of 20 under volumetric exchange ratios (VER) of 75 %, 50 %, and 25 % and mixed liquor suspended solids (MLSS) increasing from 5 to 15 g/L. The combined variation in VER and MLSS generated a gradient in food-to-microorganism radio (F/M). With increasing in biomass, COD removal efficiencies peaked at 94.4–99.3 % at 15 g MLSS/L, whereas ammonia removal efficiencies reached at 90.3–96.8 % at 12.5 g MLSS/L before declining. A VER of 25 % reduced sludge activity, while a VER of 75 % impaired floc settleability. The directed HAA community shifted in substrate flux and biomass density, centering on dominant genera such as Halomonas and Marinobacter, ultimately forming a stable microbiome.
KW - Heterotrophic ammonia assimilation
KW - High-salinity wastewater treatment
KW - Mixed liquor suspended solids
KW - Volumetric exchange ratio
UR - http://www.scopus.com/inward/record.url?scp=105027729071&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2026.133947
DO - 10.1016/j.biortech.2026.133947
M3 - Article
C2 - 41494563
AN - SCOPUS:105027729071
SN - 0960-8524
VL - 444
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 133947
ER -
