Collection of amino acids and DNA from fingerprints using hydrogels

Ward Van Helmond, Vincent O'Brien, Robin De Jong, Jan Van Esch, Sander Oldenhof*, Marcel De Puit

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)
19 Downloads (Pure)

Abstract

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualisation at a later stage using the standard method of cyanoacrylation. We will demonstrate this method to also be capable of collecting DNA from fingerprints with a 20-60% yield in comparison to using a conventional cotton swab.

Original languageEnglish
Pages (from-to)900-905
Number of pages6
JournalAnalyst
Volume143
Issue number4
DOIs
Publication statusPublished - 21 Feb 2018

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