Comparison of image reconstruction techniques for optical projection tomography

Anna K. Trull, Jelle van der Horst, Lucas J. Van Vliet, Jeroen Kalkman

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)
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Abstract

We present a comparison of image reconstruction techniques for optical projection tomography. We compare conventional filtered back projection, sinogram filtering using the frequency–distance relationship (FDR), image deconvolution, and 2D point-spread-function-based iterative reconstruction. The latter three methods aim to remove the spatial blurring in the reconstructed image originating from the limited depth of field caused by the point spread function of the imaging system. The methods are compared based on simulated data, experimental optical projection tomography data of single fluorescent beads, and high-resolution optical projection tomography imaging of an entire zebrafish larva. We demonstrate that the FDR method performs poorly on data acquired with high numerical aperture optical imaging systems. We show that the deconvolution technique performs best on highly sparse data with low signal-to-noise ratio. The point-spread-function-based reconstruction method is superior for nonsparse objects and data of high signal-to-noise ratio.

Original languageEnglish
Pages (from-to)1874-1882
Number of pages9
JournalApplied Optics
Volume57
Issue number8
DOIs
Publication statusPublished - 2018

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