Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

M. Teresa de Martino; Fabio Tonin; Victor R.L.J. Bloemendal; Ulf Hanefeld; Floris P.J.T. Rutjes; Jan C.M. van Hest

doi:10.1039/d1ra04332c

Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

M. Teresa de Martino, Fabio Tonin, Victor R.L.J. Bloemendal, Ulf Hanefeld^*, Floris P.J.T. Rutjes, Jan C.M. van Hest

^*Corresponding author for this work

BT/Biocatalysis

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3 Citations (Scopus)

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Abstract

A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.

Original language	English
Pages (from-to)	21857-21861
Journal	RSC Advances
Volume	11
Issue number	35
DOIs	https://doi.org/10.1039/d1ra04332c
Publication status	Published - 2021

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d1ra04332cFinal published version, 431 KBLicence: CC BY

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title = "Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations",

abstract = "A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.",

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T1 - Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

AU - de Martino, M. Teresa

AU - Tonin, Fabio

AU - Bloemendal, Victor R.L.J.

AU - Hanefeld, Ulf

AU - Rutjes, Floris P.J.T.

AU - van Hest, Jan C.M.

PY - 2021

Y1 - 2021

N2 - A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.

AB - A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.

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DO - 10.1039/d1ra04332c

M3 - Article

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JF - RSC Advances

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Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

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