Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

M. Teresa de Martino, Fabio Tonin, Victor R.L.J. Bloemendal, Ulf Hanefeld*, Floris P.J.T. Rutjes, Jan C.M. van Hest

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

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A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated inc-CLEnAs and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use ofc-CLEnA demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.

Original languageEnglish
Pages (from-to)21857-21861
JournalRSC Advances
Issue number35
Publication statusPublished - 2021


  • OA-Fund TU Delft


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