Correlating 3D light to 3D electron microscopy for systems biology

LM Collinson; Elizabeth Carroll; Jacob Hoogenboom

doi:10.1016/j.cobme.2017.10.006

Correlating 3D light to 3D electron microscopy for systems biology

LM Collinson, Elizabeth Carroll, Jacob Hoogenboom

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Abstract

Whilst a ‘resolution revolution’ has taken place at the macromolecular scale in both electron microscopy and light microscopy, a ‘volume revolution’ has taken place at the tissue and organism level in both imaging modalities. At both ends of the scale – resolution and volume – there are concerted efforts to link the information from light and electron microscopes through correlative workflows to link structure to function. Here, we consider the status and potential of correlative imaging in the volume domain (3D CLEM).

Original language	English
Pages (from-to)	49-55
Journal	Current Opinion in Biomedical Engineering
Volume	3
DOIs	https://doi.org/10.1016/j.cobme.2017.10.006
Publication status	Published - 15 Nov 2017

Keywords

Correlative microscopy
3D CLEM
Large scale imaging
Volume electron microscopy
Ultrastructure
Systems biology

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10.1016/j.cobme.2017.10.006

Current Opinion 3D CLEM final_revisionAccepted author manuscript, 472 KB

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KW - Correlative microscopy

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KW - Volume electron microscopy

KW - Ultrastructure

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Correlating 3D light to 3D electron microscopy for systems biology

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Keywords

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