Inconel 718 is a nickel-based superalloy commonly used in aircraft engine and nuclear applications, where components experience severe mechanical stresses. Due to the typical high temperature applications, Thermo-Mechanical Fatigue (TMF) and creep tests are common benchmarks for such applications. Additive manufacturing offers a unique way of manufacturing Inconel 718 with high degree of design freedom. However, limited knowledge exists regarding the resulting high temperature properties. The objective of this work is to evaluate creep and TMF behaviour of Inconel 718, produced by selective laser melting (SLM). A novel microstructural design, allowing for grain size control was employed in this study. The obtained functionally graded Inconel 718, exhibiting core with coarse and outside shell with fine grained microstructure, allowed for the best trade-off between creep and fatigue performance. The post heat-treatment regimens and resulting microstructures are also evaluated and its influence on creep and TMF is discussed.