TY - JOUR
T1 - Cytochrome cbb3 of Thioalkalivibrio is a Na+-pumping cytochrome oxidase
AU - Muntyan, Maria S.
AU - Cherepanov, Dmitry A.
AU - Malinen, Anssi M.
AU - Bloch, Dmitry A.
AU - Sorokin, D.
AU - Severina, Inna I.
AU - Ivashina, Tatiana V.
AU - Lahti, Reijo
AU - Muyzer, Gerard
AU - Skulachev, Vladimir P.
PY - 2015/6/23
Y1 - 2015/6/23
N2 - Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb3 cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb3 cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ionpumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.
AB - Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb3 cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb3 cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ionpumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.
KW - Alkaliphily
KW - cbb<inf>3</inf>-type oxidase
KW - Cytochrome c oxidase
KW - Sodium pumping
UR - http://www.scopus.com/inward/record.url?scp=84934890091&partnerID=8YFLogxK
U2 - 10.1073/pnas.1417071112
DO - 10.1073/pnas.1417071112
M3 - Article
C2 - 26056262
AN - SCOPUS:84934890091
SN - 0027-8424
VL - 112
SP - 7695
EP - 7700
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 25
ER -