Cytochrome cbbof Thioalkalivibrio is a Na+-pumping cytochrome oxidase

Maria S. Muntyan, Dmitry A. Cherepanov, Anssi M. Malinen, Dmitry A. Bloch, D. Sorokin, Inna I. Severina, Tatiana V. Ivashina, Reijo Lahti, Gerard Muyzer, Vladimir P. Skulachev

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Cytochrome c oxidases (Coxs) are the basic energy transducers in the respiratory chain of the majority of aerobic organisms. Coxs studied to date are redox-driven proton-pumping enzymes belonging to one of three subfamilies: A-, B-, and C-type oxidases. The C-type oxidases (cbb3 cytochromes), which are widespread among pathogenic bacteria, are the least understood. In particular, the proton-pumping machinery of these Coxs has not yet been elucidated despite the availability of X-ray structure information. Here, we report the discovery of the first (to our knowledge) sodium-pumping Cox (Scox), a cbb3 cytochrome from the extremely alkaliphilic bacterium Thioalkalivibrio versutus. This finding offers clues to the previously unknown structure of the ionpumping channel in the C-type Coxs and provides insight into the functional properties of this enzyme.

Original languageEnglish
Pages (from-to)7695-7700
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number25
DOIs
Publication statusPublished - 23 Jun 2015

Keywords

  • Alkaliphily
  • cbb<inf>3</inf>-type oxidase
  • Cytochrome c oxidase
  • Sodium pumping

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