Designing biocatalytic redox reactions with oxidoreductases for organic chemistry

Marine Rauch

doi:10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de

Designing biocatalytic redox reactions with oxidoreductases for organic chemistry

Marine Rauch

BT/Biocatalysis

Research output: Thesis › Dissertation (TU Delft)

291 Downloads (Pure)

Abstract

The focus of this thesis is to identify improvements of oxidoreductase reactions to bring them to preparative scale. Three approaches were studied: (1) photoregeneration of oxidised nicotinamide cofactors with LEDs as light source and flavins, (2) direct regeneration of Old Yellow Enzymes with light or metals, (3) heme-thiolate enzyme peroxygenases in neat substrate conditions.
Flavins are the main actors of the first two approaches. These redox cofactors have been chosen for their photocatalytic properties as a photosensitiser and as a cofactor in the so-called flavoproteins.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	Delft University of Technology
Supervisors/Advisors	Hollmann, F., Supervisor Arends, Isabel, Supervisor Paul, C.E., Advisor
Award date	27 Mar 2020
Print ISBNs	978-94-6384-112-2
DOIs	https://doi.org/10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de
Publication status	Published - 7 Feb 2020

Access to Document

10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de

PhD thesis Marine C.R. Rauch finalFinal published version, 3.88 MB

Cite this

@phdthesis{afcbf229f6ba4b81a5616a0a61dc74de,

title = "Designing biocatalytic redox reactions with oxidoreductases for organic chemistry",

abstract = "The focus of this thesis is to identify improvements of oxidoreductase reactions to bring them to preparative scale. Three approaches were studied: (1) photoregeneration of oxidised nicotinamide cofactors with LEDs as light source and flavins, (2) direct regeneration of Old Yellow Enzymes with light or metals, (3) heme-thiolate enzyme peroxygenases in neat substrate conditions.Flavins are the main actors of the first two approaches. These redox cofactors have been chosen for their photocatalytic properties as a photosensitiser and as a cofactor in the so-called flavoproteins.",

author = "Marine Rauch",

year = "2020",

month = feb,

day = "7",

doi = "10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de",

language = "English",

isbn = "978-94-6384-112-2",

type = "Dissertation (TU Delft)",

school = "Delft University of Technology",

}

TY - THES

T1 - Designing biocatalytic redox reactions with oxidoreductases for organic chemistry

AU - Rauch, Marine

PY - 2020/2/7

Y1 - 2020/2/7

N2 - The focus of this thesis is to identify improvements of oxidoreductase reactions to bring them to preparative scale. Three approaches were studied: (1) photoregeneration of oxidised nicotinamide cofactors with LEDs as light source and flavins, (2) direct regeneration of Old Yellow Enzymes with light or metals, (3) heme-thiolate enzyme peroxygenases in neat substrate conditions.Flavins are the main actors of the first two approaches. These redox cofactors have been chosen for their photocatalytic properties as a photosensitiser and as a cofactor in the so-called flavoproteins.

AB - The focus of this thesis is to identify improvements of oxidoreductase reactions to bring them to preparative scale. Three approaches were studied: (1) photoregeneration of oxidised nicotinamide cofactors with LEDs as light source and flavins, (2) direct regeneration of Old Yellow Enzymes with light or metals, (3) heme-thiolate enzyme peroxygenases in neat substrate conditions.Flavins are the main actors of the first two approaches. These redox cofactors have been chosen for their photocatalytic properties as a photosensitiser and as a cofactor in the so-called flavoproteins.

U2 - 10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de

DO - 10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de

M3 - Dissertation (TU Delft)

SN - 978-94-6384-112-2

ER -

Designing biocatalytic redox reactions with oxidoreductases for organic chemistry

Abstract

Access to Document

Fingerprint

Cite this