Designing biocatalytic redox reactions with oxidoreductases for organic chemistry

The focus of this thesis is to identify improvements of oxidoreductase reactions to bring them to preparative scale. Three approaches were studied: (1) photoregeneration of oxidised nicotinamide cofactors with LEDs as light source and flavins, (2) direct regeneration of Old Yellow Enzymes with light or metals, (3) heme-thiolate enzyme peroxygenases in neat substrate conditions.
Flavins are the main actors of the first two approaches. These redox cofactors have been chosen for their photocatalytic properties as a photosensitiser and as a cofactor in the so-called flavoproteins.