Detection of comammox bacteria in full-scale wastewater treatment bioreactors using tag-454-pyrosequencing

Alejandro Gonzalez-Martinez, Alejandro Rodriguez-Sanchez, M. C M van Loosdrecht, Jesus Gonzalez-Lopez, Riku Vahala

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Abstract

The nitrogen cycle has been expanded with the recent discovery of Nitrospira strains that can conduct complete ammonium oxidation (commamox). Their importance in the nitrogen cycle within engineered ecosystems has not yet been analyzed. In this research, the community structure of the Bacteria domain of six full-scale activated sludge systems and three autotrophic nitrogen removal systems in the Netherlands and China has been investigated through tag-454-pyrosequencing. The phylogenetic analyses conducted in the present study showed that just a few of the Nitrospira sequences found in the bioreactors were comammox. Multivariate redundancy analysis of nitrifying genera showed an outcompetition of Nitrosomonas and non-comammox Nitrospira. Operational data from the bioreactors suggested that comammox could be favored at low temperature, low nitrogen substrate, and high dissolved oxygen. The non-ubiquity and low relative abundance of comammox in full-scale bioreactors suggested that this phylotype is not very relevant in the nitrogen cycle in wastewater treatment plants.

Original languageEnglish
Pages (from-to)25501-25511
Number of pages11
JournalEnvironmental Science and Pollution Research
Volume23
Issue number24
DOIs
Publication statusPublished - 1 Dec 2016

Keywords

  • Activated sludge
  • Comammox
  • Nitrogen
  • Phylogenetics
  • Pyrosequencing

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