Detection of Schistosoma haematobium using lensless imaging and flow cytometry, a proof of principle study

Tope Agbana, Patrick Nijman, M.D.B. Hoeboer, D.N. van Grootheest, Angela van Diepen, Lisette van Lieshout, Jan-Carel Diehl, Michel Verhaegen, Gleb Vdovin

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

8 Citations (Scopus)
148 Downloads (Pure)


We present a simple method for the diagnosis of urinary schistosomiasis using an in-line lensless holographic microscope combined with flow cytometry technique. Using simple image processing algorithms and binary image classifier, our system provides automated detection of Schistosoma haematobium eggs in infected urine samples. Registered hologram is reconstructed by applying backpropagation from sensor to sample plane and reconstructed image is automatically analysed for the presence of S. haematobium eggs. Designed for use in a resource-poor laboratory setting, our proposed method has been implemented using a Raspberry Pi computer. From pre-clinical test performed with human urine samples spiked with S. haematobium eggs (approximately 200 eggs per 12 ml of urine), we achieved a sensitivity and specificity of 50.6% and 98.6% respectively. Our proposed method requires no complex sample preparation methods making the system simple to operate and useable in point-of-care diagnosis of urinary schistosomiasis.This method can be optimized to complement existing diagnostic procedures for the detection of S. haematobium eggs and can be deployed to inaccessible remote areas.
Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationOptical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics
EditorsGerard L. Coté
Place of PublicationBellingham, WA, USA
Number of pages10
ISBN (Print)978-1-5106-3257-8
Publication statusPublished - 2020
EventSPIE BiOS: Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics - The Moscone Center, San Francisco,California, United States
Duration: 1 Feb 20206 Feb 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceSPIE BiOS
Country/TerritoryUnited States
CitySan Francisco,California
Internet address


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