Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Jochem N.A. Vink, Koen J.A. Martens, Marnix Vlot, Rebecca E. McKenzie, Cristóbal Almendros, Boris Estrada Bonilla, Daan J.W. Brocken, Johannes Hohlbein, Stan J.J. Brouns

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

Original languageEnglish
Pages (from-to)39-50.e10
JournalMolecular Cell
Volume77
Issue number1
DOIs
Publication statusPublished - 2020

Keywords

  • arms race
  • Cascade
  • CRISPR-Cas
  • PALM
  • PAM
  • photo-activation localization microscopy
  • single molecule
  • single-particle tracking
  • target search

Fingerprint Dive into the research topics of 'Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism'. Together they form a unique fingerprint.

  • Cite this