Discovery and structural characterization of fucosylated oligomannosidic N-glycans in mushrooms

Josephine Grass, Martin Pabst, Daniel Kolarich, Gerald Pöltl, Renaud Léonard, Lothar Brecker, Friedrich Altmann

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

L-Fucose is a common constituent of Asn-linked glycans in vertebrates, invertebrates, and plants, but in fungal glycoproteins, fucose has not been found so far. However, by mass spectrometry we detected N-glycans and O-glycans containing one to six deoxyhexose residues in fruit bodies of several basidiomycetes. The N-glycans of chanterelles (Cantharellus cibarius) contained a deoxyhexose chromatographically identical to fucose and sensitive to α-L-fucosidase. Analysis of individual glycan species by tandem MS, glycosidase digestion, and finally 1H NMR revealed the presence of L-fucose in α1,6-linkage to an α1,6-mannose of oligomannosidic N-glycans. The substitution by α1,6-mannose of α1,2-mannosyl residues of the canonical precursor structure was yet another hitherto unknown modification. No indication for the occurrence of yet other modifications, e.g. bisecting N-acetylglucosamine, was seen. Besides fucosylated N-glycans, short O-linked mannan chains substituted with fucose were present on chanterelle proteins. Although undiscovered so far, L-fucose appears to represent a prominent feature of protein-linked glycans in the fungal kingdom.

Original languageEnglish
Pages (from-to)5977-5984
Number of pages8
JournalJournal of Biological Chemistry
Volume286
Issue number8
DOIs
Publication statusPublished - 25 Feb 2011

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