TY - JOUR
T1 - DNA-membrane complex formation during electroporation is DNA size-dependent
AU - Sachdev, Shaurya
AU - Feijoo Moreira, Sara
AU - Keehnen, Yasmine
AU - Rems, Lea
AU - Kreutzer, Michiel T.
AU - Boukany, Pouyan E.
PY - 2020
Y1 - 2020
N2 - Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to investigate the effect of DNA size on DNA-membrane interaction during electroporation, cells are electro-pulsed with DNA molecules; 15 bp, 25 bp, 50 bp, 100 bp and 1000 bp (bp = base pairs). Within the experimental parameter space, DNA-membrane complexes or DNA aggregates are observed at the cell membrane for DNA molecules containing 25 or more base pairs. No aggregates are observed for DNA molecules containing 15 bp. For all DNA sizes, direct access to the cytoplasm is observed, however the amount translocated decays with the size. The observed dependency of DNA aggregate formation on the size of the DNA molecules is consistent with the Onsager's theory of condensation of anisotropic rod-like molecules.
AB - Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to investigate the effect of DNA size on DNA-membrane interaction during electroporation, cells are electro-pulsed with DNA molecules; 15 bp, 25 bp, 50 bp, 100 bp and 1000 bp (bp = base pairs). Within the experimental parameter space, DNA-membrane complexes or DNA aggregates are observed at the cell membrane for DNA molecules containing 25 or more base pairs. No aggregates are observed for DNA molecules containing 15 bp. For all DNA sizes, direct access to the cytoplasm is observed, however the amount translocated decays with the size. The observed dependency of DNA aggregate formation on the size of the DNA molecules is consistent with the Onsager's theory of condensation of anisotropic rod-like molecules.
KW - DNA aggregation
KW - DNA size
KW - DNA-membrane complex formation
KW - Electropermeabilization
KW - Electroporation
KW - Gene electrotransfer
UR - http://www.scopus.com/inward/record.url?scp=85075477985&partnerID=8YFLogxK
U2 - 10.1016/j.bbamem.2019.183089
DO - 10.1016/j.bbamem.2019.183089
M3 - Article
C2 - 31678021
AN - SCOPUS:85075477985
SN - 0005-2736
VL - 1862
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
M1 - 183089
ER -