@inbook{e8f01950981d4af5b8aece1e877c178b,
title = "In vitro reconstitution of dynamic co-organization of microtubules and actin filaments in emulsion droplets",
abstract = "In vitro (or cell-free) reconstitution is a powerful tool to study the physical basis of cytoskeletal organization in eukaryotic cells. Cytoskeletal reconstitution studies have mostly been done for individual cytoskeleton systems in unconfined 3D or quasi-2D geometries, which lack complexity relative to a cellular environment. To increase the level of complexity, we present a method to study co-organization of two cytoskeletal components, namely microtubules and actin filaments, confined in cell-sized water-in-oil emulsion droplets. We show that centrosome-nucleated dynamic microtubules can be made to interact with actin filaments through a tip-tracking complex consisting of microtubule end-binding proteins and an actin-microtubule cytolinker. In addition to the protocols themselves, we discuss the optimization steps required in order to build these more complex in vitro model systems of cytoskeletal interactions.",
keywords = "Confinement, Cytolinkers, Cytoskeleton, In vitro reconstitution, Microtubule dynamic instability, Self-organization",
author = "Vendel, {Kim J.A.} and Celine Alkemade and Nemo Andrea and Koenderink, {Gijsje H.} and Marileen Dogterom",
year = "2020",
doi = "10.1007/978-1-0716-0219-5_5",
language = "English",
isbn = "978-1-0716-0218-8",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "53--75",
editor = "Helder Maiato",
booktitle = "Cytoskeleton Dynamics",
}