TY - JOUR
T1 - Effect of aliphatic diamine spacer length on enzymatic performance of myrosinase immobilized on chitosan microsphere and its application for sulforaphene production
AU - Zhang, Jie
AU - Feng, Chunrong
AU - Tan, Xuemei
AU - Hagedoorn, Peter Leon
AU - Gu, Changhong
AU - Xu, Hui
AU - Zhou, Xing
PY - 2019
Y1 - 2019
N2 -
Glucosinolates can be hydrolyzed by the enzyme commonly known as myrosinase (E.C. 3.2.1.147)to a variety of biological compounds. Myrosinase (MYR)has been immobilized through the flexible spacers of different length on cross-linked chitosan resin (CCR). Ethylene diamine, hexamethylenediamine and decamethylene-diamine have been separately used as spacers. The influence of the flexible spacer length on the immobilized MYR (IMYR)properties were evaluated. The optimum pH and V
max
of IMYR linearly increase with the flexible spacer length, and the optimum temperature and K
m
of IMYR show an opposite trend. The recyclability of IMYR was good, with 90% recovery of activity after 10 cycles and 80% recovery after 30 cycles. IMYR was highly stable under storage conditions, with 95% recovery of activity after one year storage at 4 °C. The IMYR with the longest flexible spacer, decamethylene-diamine, was used as a biocatalyst for sulforaphene production. The overall hydrolysis ratio of glucoraphenin was 93.25 ± 0.91% and the activity of DDMCCR-IMYR remained 95% after 10 days of continuous use.
AB -
Glucosinolates can be hydrolyzed by the enzyme commonly known as myrosinase (E.C. 3.2.1.147)to a variety of biological compounds. Myrosinase (MYR)has been immobilized through the flexible spacers of different length on cross-linked chitosan resin (CCR). Ethylene diamine, hexamethylenediamine and decamethylene-diamine have been separately used as spacers. The influence of the flexible spacer length on the immobilized MYR (IMYR)properties were evaluated. The optimum pH and V
max
of IMYR linearly increase with the flexible spacer length, and the optimum temperature and K
m
of IMYR show an opposite trend. The recyclability of IMYR was good, with 90% recovery of activity after 10 cycles and 80% recovery after 30 cycles. IMYR was highly stable under storage conditions, with 95% recovery of activity after one year storage at 4 °C. The IMYR with the longest flexible spacer, decamethylene-diamine, was used as a biocatalyst for sulforaphene production. The overall hydrolysis ratio of glucoraphenin was 93.25 ± 0.91% and the activity of DDMCCR-IMYR remained 95% after 10 days of continuous use.
KW - Chitosan
KW - Diamine
KW - Glucoraphenin
KW - Immobilization
KW - Myrosinase
KW - Sulforaphene
UR - http://www.scopus.com/inward/record.url?scp=85065447051&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2019.04.023
DO - 10.1016/j.jbiotec.2019.04.023
M3 - Article
C2 - 31042585
AN - SCOPUS:85065447051
VL - 299
SP - 79
EP - 85
JO - Journal of Biotechnology (online)
JF - Journal of Biotechnology (online)
SN - 1873-4863
ER -