Effect of short term external perturbations on bacterial ecology and activities in a partial nitritation and anammox reactor

Sha Wu, Ananda S. Bhattacharjee, David G. Weissbrodt, Eberhard Morgenroth, Ramesh Goel

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

This research investigated the short term effects of temperature changes (lasting 2–4 weeks each) from 35 ± 2 °C to 21 ± 2 °C and 13 ± 2 °C and sulfide toxicity on partial nitrification-anammox (PN/A) system. Temperatures below 20 °C and sulfide content as low as 5 mgS L−1 affected both aerobic and anaerobic catabolic activities of ammonia oxidation and the expression of related functional gene markers. The activity of AOB was inversely correlated with ammonium monooxygenase (amoA) gene expression. In contrast, the activity of AMX bacteria was positively correlated with the expression of their hydrazine synthase (hzsA) gene. Although the overall activities of AMX bacteria decreased at lower temperatures, the AMX bacteria were still active at the low temperatures. The inverse correlation between amoA gene expressions and the corresponding AOB activities was surprising. 16S rDNA based high throughput amplicon sequencing revealed the dominance of Chloroflexi, Planctomycetes and Proteobacteria phyla the distribution of which changed with temperature changes.

Original languageEnglish
Pages (from-to)527-535
Number of pages9
JournalBioresource Technology
Volume219
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • amoA gene and hzsA gene expressions
  • Nitritation/anammox
  • Sulfide
  • Temperature

Fingerprint Dive into the research topics of 'Effect of short term external perturbations on bacterial ecology and activities in a partial nitritation and anammox reactor'. Together they form a unique fingerprint.

  • Cite this