Efficient Aerobic Oxidation of trans-2-Hexen-1-ol using the Aryl Alcohol Oxidase from Pleurotus eryngii

T. P. de Almeida, M. M.C.H. van Schie, A. Ma, F. Tieves, S. H.H. Younes, E. Fernández-Fueyo, I. W.C.E. Arends, A. Riul, F. Hollmann

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Abstract

The selective oxidation of trans-2-hexen-1-ol to the corresponding aldehyde using a recombinant aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. Especially using the two liquid phase system to overcome solubility and product inhibition issues enabled to achieve more than 2.200.000 catalytic turnovers for the production enzyme as well as molar product concentrations, pointing towards an economic feasible reaction.

Original languageEnglish
Pages (from-to)2668-2672
Number of pages5
JournalAdvanced Synthesis and Catalysis
Volume361
Issue number11
DOIs
Publication statusPublished - 2019

Keywords

  • biocatalysis
  • oxidase
  • oxidation reactions
  • two liquid phase system

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