Abstract
NADH mimics (mNADHs) have been shown to accelerate and orthogonally activate ene reductase-catalyzed reactions. However, existing regeneration methods of NAD(P)H fail for mNADHs. Catalysis with artificial metalloenzymes based on streptavidin (Sav) variants and a biotinylated iridium cofactor enable mNADH regeneration with formate. This regeneration can be coupled with ene reductase-catalyzed asymmetric reduction of α,β-unsaturated compounds, because of the protective compartmentalization of the organometallic cofactor. With 10 mol% mNAD+, a preparative scale reaction (>100 mg) gave full conversion with 98% ee, where TTNs reached 2000, with respect to the Ir cofactor under ambient atmosphere in aqueous medium.
Original language | English |
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Pages (from-to) | 3553-3557 |
Journal | ACS Catalysis |
Volume | 6 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- artificial metalloenzyme
- asymmetric catalysis
- biocatalysis
- iridium
- renewable source