Electochemical detection of enzyme kinetics using a nanofluidic thin layer cell device.

ED Goluch, N Wongrajit, PS Singh, AWJW Tepper, HA Heering, GW Canters, SG Lemay

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

Abstract

ABSTRACT Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol. KEYWORDS: Electrochemistry, Redox Cycling, Enzyme Kinetics, Nanofluidics
Original languageEnglish
Title of host publicationUnknown
Editors MicroTAS 2010
Place of PublicationGroningen
Publishers.n.
Pages482-484
Number of pages3
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2010) - Groningen
Duration: 3 Oct 20107 Oct 2010

Publication series

Name
PublisherSN

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2010)
Period3/10/107/10/10

Keywords

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