@inproceedings{66b427c36d274d98be4c4daa8e3e8ebe,
title = "Electochemical detection of enzyme kinetics using a nanofluidic thin layer cell device.",
abstract = "ABSTRACT Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol. KEYWORDS: Electrochemistry, Redox Cycling, Enzyme Kinetics, Nanofluidics",
keywords = "Geen BTA classificatie",
author = "ED Goluch and N Wongrajit and PS Singh and AWJW Tepper and HA Heering and GW Canters and SG Lemay",
year = "2010",
language = "English",
publisher = "s.n.",
pages = "482--484",
editor = "{MicroTAS 2010}",
booktitle = "Unknown",
note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2010) ; Conference date: 03-10-2010 Through 07-10-2010",
}