Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device.

ED Goluch, N Wongrajit, PS Singh, AWJW Tepper, HA Heering, GW Canters, SG Lemay

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

Abstract

ABSTRACT We have developed a lithography-based nanofluidic device for sensitive electrochemical detection applications. The device consists of a solution-filled channel bounded by parallel electrodes that are separated by ~50 nm. Redox cycling at the two electrodes is used to amplify the electrochemical signals from molecules in the volume between the electrodes, and can lead to dramatic improvements in sensitivity and/or selectivity. Interestingly, an important limit to sensitivity is placed by statistical fluctuations in the number of molecules present in the device, a consequence of its extremely small volume (a few femtolitres). In this Proceedings article, we focus on the consequences of these statistical fluctuations on the signal-to-noise ratio of electrochemical sensors based on redox cycling. KEYWORDS: Nanofluidics, electrochemistry, sensing, number fluctuations, mesoscopic.
Original languageEnglish
Title of host publicationUnknown
Editors SN
Place of PublicationGroningen
PublisherGroningen
Pages482-484
Number of pages3
Publication statusPublished - 2010

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PublisherGroningen

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