Electrokinetic Concentration of DNA polymers in Nanofluidic channels

D Stein, Z Deurvorst, FHJ Heyden, WJA Koopmans, A Gabel, C Dekker

Research output: Contribution to journalArticleScientificpeer-review

65 Citations (Scopus)

Abstract

DNA molecules can be concentrated in a narrow region of a nanochannel when driven electrokinetically in submillimolar salt solutions. Transport experiments and theoretical modeling reveal the interplay of electrophoresis, electro-osmosis, and the unique statistical properties of confined polymers that lead to DNA aggregation. A finite conductance through the bulk of the device also plays a crucial role by influencing the electric fields in the nanochannel. We build on this understanding by demonstrating how a nanofluidic device with integrated electrodes can preconcentrate DNA at selected locations and at physiological salt concentrations that are relevant to lab-on-a-chip applications.
Original languageEnglish
Pages (from-to)765-772
Number of pages8
JournalNano Letters: a journal dedicated to nanoscience and nanotechnology
Volume10
Issue number3
Publication statusPublished - 2010

Keywords

  • academic journal papers
  • CWTS JFIS >= 2.00

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