Electroporation of biomimetic vesicles

Dayinta Perrier

Research output: ThesisDissertation (TU Delft)

117 Downloads (Pure)

Abstract

Electroporation is a popular technique to permeabilize the membrane for different purposes such as medical treatments, food processing and biomass processing. In this thesis, we use the bottom-up approach to unravel the role of specific cellular components in the electroporation of cellular membranes. We have studied the role of the gel-phase domains in the membrane and the contribution of the actin-cortex during electroporation. In order to do so, we have prepared binary-phase vesicles, containing fluid- and gelphase lipids, and actin-cortex encapsulated vesicles. Consequently, the electroporation mechanisms of these two samples provide systematic insight in the electroporation mechanism of a single cell.
Original languageEnglish
Awarding Institution
  • Delft University of Technology
Supervisors/Advisors
  • Kreutzer, M.T., Supervisor
  • Boukany, P., Advisor
Award date26 Nov 2018
Print ISBNs978.90.8593.371.7
DOIs
Publication statusPublished - 2018

Keywords

  • electroporation
  • electropermeabilization
  • electric field, lipid vesicles
  • giant unilamellar vesicles
  • GUVs
  • gel-phase lipids
  • fluid-phase lipids
  • binary-phase
  • actin network

Fingerprint Dive into the research topics of 'Electroporation of biomimetic vesicles'. Together they form a unique fingerprint.

Cite this