Electroporation is a popular technique to permeabilize the membrane for different purposes such as medical treatments, food processing and biomass processing. In this thesis, we use the bottom-up approach to unravel the role of specific cellular components in the electroporation of cellular membranes. We have studied the role of the gel-phase domains in the membrane and the contribution of the actin-cortex during electroporation. In order to do so, we have prepared binary-phase vesicles, containing fluid- and gelphase lipids, and actin-cortex encapsulated vesicles. Consequently, the electroporation mechanisms of these two samples provide systematic insight in the electroporation mechanism of a single cell.
|Award date||26 Nov 2018|
|Publication status||Published - 2018|
- electric field, lipid vesicles
- giant unilamellar vesicles
- gel-phase lipids
- fluid-phase lipids
- actin network