Abstract
Electroporation is a popular technique to permeabilize the membrane for different purposes such as medical treatments, food processing and biomass processing. In this thesis, we use the bottom-up approach to unravel the role of specific cellular components in the electroporation of cellular membranes. We have studied the role of the gel-phase domains in the membrane and the contribution of the actin-cortex during electroporation. In order to do so, we have prepared binary-phase vesicles, containing fluid- and gelphase lipids, and actin-cortex encapsulated vesicles. Consequently, the electroporation mechanisms of these two samples provide systematic insight in the electroporation mechanism of a single cell.
| Original language | English |
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| Awarding Institution |
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| Award date | 26 Nov 2018 |
| Print ISBNs | 978.90.8593.371.7 |
| DOIs | |
| Publication status | Published - 2018 |
Keywords
- electroporation
- electropermeabilization
- electric field, lipid vesicles
- giant unilamellar vesicles
- GUVs
- gel-phase lipids
- fluid-phase lipids
- binary-phase
- actin network