Enantioselective Sulfoxidation of Thioanisole by Cascading a Choline Oxidase and a Peroxygenase in the Presence of Natural Deep Eutectic Solvents

Yongru Li, Yunjian Ma, Peilin Li, Xizhen Zhang, Doris Ribitsch, Miguel Alcalde, Frank Hollmann, Yonghua Wang

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)
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Abstract

A bienzymatic cascade for selective sulfoxidation is presented. The evolved recombinant peroxygenase from Agrocybe aegeritra catalyses the enantioselective sulfoxidation of thioanisole whereas the choline oxidase from Arthrobacter nicotianae provides the H2 O2 necessary via reductive activation of ambient oxygen. The reactions are performed in choline chloride-based deep eutectic solvents serving as co-solvent and stoichiometric reductant at the same time. Very promising product concentrations (up to 15 mM enantiopure sulfoxide) and catalyst performances (turnover numbers of 150,000 and 2100 for the peroxygenase and oxidase, respectively) have been achieved.

Original languageEnglish
Pages (from-to)254-257
JournalChemPlusChem
Volume85
Issue number1
DOIs
Publication statusPublished - 2020

Keywords

  • biocatalysis
  • enantioselectivity
  • natural deep eutectic solvents
  • peroxygenases
  • sulfoxidation

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